Iowa House Republicans passed a bill Monday to ban transgender girls from women’s sports, the most expansive proposal yet in an ongoing effort to regulate transgender student athletes.

House File 2416 would prohibit transgender girls from participating in girls’ sports in Iowa K-12 schools and colleges. That means any students who are “biological males,” regardless of their gender identity, would not be allowed to compete against biological females.

“Biological males, who have significant physiological advantages, do not get to play female sports,” said Rep. Skyler Wheeler, who led debate on the proposal. “It’s very clear.”

The bill would apply to public and nonpublic K-12 schools, public colleges governed by the Board of Regents, community colleges and any other colleges or universities that are members of the National Collegiate Athletic Association (NCAA), National Association of Intercollegiate Athletics (NAIA) or the National Junior College Athletic Association (NJCAA).

Republican proponents of the legislation, including Gov. Kim Reynolds, argued it is physically unfair to allow transgender women to play alongside biological females.